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Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice* * Extracted from the thesis "Toxicity of polymyxin B in LLC-PK1 cells and the heme oxygenase-1 enzyme", School of Nursing, Universidade de São Paulo, 2008

La Nefrotoxicidad De Polimixina B: Estudio Experimental En Las Células Y Implicaciones Para La Práctica De Enfermería

The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.


Polymyxin B
; Anti-bacterial agents
; Acute kidney injury
; LLC-PK1 cells
; Nursing care



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