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vol.22 número3Efeito da saturação por bases sobre a atividade de fosfatases em um solo de tabuleiro costeiro cultivado com citros. I. Correlações entre a atividade enzimática e as diferentes características do solo alteradas pela calagemNitrogênio da biomassa microbiana, em solo sob diferentes sistemas de manejo, estimado por métodos de fumigação índice de autoresíndice de assuntospesquisa de artigos
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Revista Brasileira de Ciência do Solo

versão On-line ISSN 1806-9657

Resumo

FERNANDES, M. F. et al. Effect of base saturation on phosphatase activities from a coastal tableland soil cultivated with orange tree. II. Kinetic constants. Rev. Bras. Ciênc. Solo [online]. 1998, vol.22, n.3, pp.403-409. ISSN 1806-9657.  http://dx.doi.org/10.1590/S0100-06831998000300005.

The kinetic constants Kmapp and Vmaxapp of phosphatase activity from a coastal tableland soil grown with orange trees, submitted to different base saturation levels (32.0; 45.0; 64.0 and 76.6%), were determined. These determinations were made in optimum pH values for alkaline and acid phosphatases and in the pH values obtained for each base saturation level. Phosphatase activity has followed Michaelis-Menten kinetics, regardless the soil base saturation levels or pH values used for enzymatic activity evaluation. Increases in the soil bases saturation resulted in lower Kmapp values for both enzymes, while Vmaxapp was increased for alkaline phosphatase and did not show significant variation for acid phosphatase. Decreases in Kmapp and increases in Vmaxapp values in response to increasing soil bases saturation were also observed for phosphatases evaluated at pH values found at each soil base saturation level. The most remarkable changes in Kmapp and Vmaxapp in response to soil base saturation were the result of shifts in pH values.

Palavras-chave : enzymatic kinetic; enzyme; phosphatase; coastal tableland; citrus; soil base saturation; liming.

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