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Revista Brasileira de Ciência do Solo

versão On-line ISSN 1806-9657


LUPATINI, Manoeli; MELLO, Andrea Hentz de  e  ANTONIOLLI, Zaida Inês. Ribosomal dna characterization of Scleroderma UFSMSc1 of Eucalyptus grandis W. Hill ex-maiden. Rev. Bras. Ciênc. Solo [online]. 2008, vol.32, n.spe, pp.2677-2682. ISSN 1806-9657.

The ribossomal deoxyribonucleic acid (rDNA) represents an important tool in the characterization of fungi polymorphism. There are many copies of rDNA arranged in non encoded spaces. These copies are highly preserved in fungus species. The purpose of this study was to investigate the Internal Transcribed Spacer (ITS) region and to analyze the differences in the sequence polymorphism of the ITS region in the fungus Scleroderma UFSMSc1 with sequences of Scleroderma and Pisolithus isolates from the GenBank database. The DNA of the Scleroderma UFSMSc1 isolate was extracted with CTAB solution. PCR reactions were carried out with the universal primers ITS1 and ITS4. The amplified products were purified and sequenced. A simple band with a fragment of approximately 650 base pairs was observed in the ITS region of the fungus. The formation of grouping with Scleroderma species was observed in the sequence analysis of this region in comparison with entries of the GenBank database. The results confirmed this technique for Scleroderma species identification, since these fungi are not easily identified by morphological characters.

Palavras-chave : DNA characterization; PCR; Scleroderma.

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