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Pesquisa Agropecuária Brasileira

Print version ISSN 0100-204XOn-line version ISSN 1678-3921

Abstract

FREITAS, LORETA BRANDÃO DE; JERUSALINSKY, LEANDRO; BONATTO, SANDRO LUIS  and  SALZANO, FRANCISCO MAURO. Extreme homogeneity among Brazilian wheat genotypes determined by RAPD markers. Pesq. agropec. bras. [online]. 2000, vol.35, n.11, pp.2255-2260. ISSN 0100-204X.  https://doi.org/10.1590/S0100-204X2000001100018.

Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 14 genotypes of Brazilian wheat (Triticum aestivum L.), using a set of 50 random 10mer primers. A total of 256 reproducibly scorable DNA amplification products were obtained from 48 of the primers, 83% of which were polymorphic. Genetic distances among genotypes were calculated and a dendrogram and a principal coordinates analysis showing the genetic relationships among them were obtained. Despite the low variability found (average genetic distance of 27%), two groups of genotypes could be identified, which probably reflect how they were formed. Studies such as this one may be important in the planning and development of future improvement programs for this plant species.

Keywords : Triticum aestivum; genetic variation; polymorphism; breeding methods.

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