Pesquisa Agropecuária Brasileira
Print version ISSN 0100-204X
MATSUMOTO, Kazumitsu et al. Cryopreservation and somatic embryogenesis of Dimocarpus longan calli. Pesq. agropec. bras. [online]. 2004, vol.39, n.12, pp. 1261-1263. ISSN 0100-204X. http://dx.doi.org/10.1590/S0100-204X2004001200014.
Cryoprotectors on cryopreservation and sucrose on somatic embryogenesis of Dimocarpus longan calli were evaluated. The post-thaw calli were cultivated on multiplication medium, and the obtained fresh matter mass was measured. In order to obtain somatic embryos, calli and pro-embryonic masses were transferred into culture medium containing different concentrations of sucrose. Among the cryoprotectors, the mixture of 5% glycerol + 5% dimethylsulfoxide provided the largest quantity of calli fresh matter. The highest number of embryos was obtained on the culture medium with 50 g L-1 sucrose. The results show that Dimocarpus longan calli can be cryopreserved and plantlets be obtained.
Keywords : Sapindaceae; glycerol; dimethylsulfoxide; micropropagation.