Pesquisa Agropecuária Brasileira
versión On-line ISSN 1678-3921
MEDEIROS, Patrícia Teles et al. Selection and characterization of Bacillus thuringiensis strains effective to control the diamondback moth Plutella xylostella. Pesq. agropec. bras. [online]. 2005, vol.40, n.11, pp.1145-1148. ISSN 1678-3921. http://dx.doi.org/10.1590/S0100-204X2005001100014.
The aim of this work was to select and characterize the most toxic Bacillus thuringiensis strains, from the Germplasm Bank of Bacillus spp. of Empresa Brasileira de Pesquisa Agropecuária, against Plutella xylostella. Strains were characterized by morphological, biochemical and molecular methods. It was observed that seven out of the 203 strains tested showed high toxicity compared to the standard used B. thuringiensis subsp. kurstaki (HD-1), which showed 100% mortality. Selected strains showed features described for lepidoptera regarding the protein of 130 kDa and 65 kDa; profile and features were obtained through the PCR reactions, making possible to identify the presence of cry1 and cry2 genes. Moreover, the scanning electron microscopy showed the bipiramydal, cubed and round crystal forms. The selected strains offer new perspectives to control P. xylostella.
Palabras clave : protein cristals; entomopathogenic bacteria; biopesticides.