Pesquisa Agropecuária Brasileira
versão impressa ISSN 0100-204X
versão On-line ISSN 1678-3921
LACAVA, Paulo Teixeira; ANDREOTE, Fernando Dini; ARAUJO, Welington Luiz e AZEVEDO, João Lúcio. Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE. Pesq. agropec. bras. [online]. 2006, vol.41, n.4, pp.637-642. ISSN 0100-204X. http://dx.doi.org/10.1590/S0100-204X2006000400013.
The aim of this work was to characterize endophytic bacterial community of assintomatic (escape) and Citrus Variegated Chlorosis (CVC)-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE) technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.
Palavras-chave : Citrus sinensis; Xylella fastidiosa; Methylobacterium; endophytic bacteria.