Pesquisa Agropecuária Brasileira
versión impresa ISSN 0100-204X
versión On-line ISSN 1678-3921
JESUS, Onildo Nunes de et al. Molecular differentiation of elite banana cultivars. Pesq. agropec. bras. [online]. 2006, vol.41, n.12, pp.1739-1748. ISSN 0100-204X. http://dx.doi.org/10.1590/S0100-204X2006001200008.
The objective of this work was to characaterize elite banana genotypes, and those recommended, using RAPD and microsatellites. Forty-seven RAPD primers and 34 microsatellites primers were used. A contamination essay using the AGMI 24-25 primer was also carried out. Tropical variety was considered the standard sample and the Caipira and Prata Graúda, contaminants. Markers were able to separate the cultivars according to their origin and genomic constitution as well as defined molecular profiles for some of the cultivars evaluated. Garantida, Preciosa and Pacovan Ken cultivars presented genetic similarity with both markers. The AGMI 24-25 primer demonstrated high capacity to discriminate the genotypes in the contamination essay.
Palabras clave : Musa spp.; genetic certification; varietal protection; fingerprinting; molecular markers.