Pesquisa Agropecuária Brasileira
versión On-line ISSN 1678-3921
AMORIM, Edson Perito et al. Genetic variability estimated in banana diploids through microsatellite markers. Pesq. agropec. bras. [online]. 2008, vol.43, n.8, pp. 1045-1052. ISSN 1678-3921. http://dx.doi.org/10.1590/S0100-204X2008000800014.
The objective of this work was to estimate the genetic diversity between 38 banana diploids from the banana breeding program of Embrapa Mandioca e Fruticultura Tropical (Embrapa Cassava and Tropical Fruits), including improved hybrids, cultivated and wild species, using fifteen SSR markers. Genetic similarities were utilized to cluster genotypes through UPGMA, based on Jaccard coefficient. The average number of alleles per primer was 7.53, with a total of 113 alleles identified. The average similarity was 0.22, and ranged from 0.028 to 0.48, indicating the existence of genetic variability between genotypes. The clustering analysis based on microsatellite polymorphism could not completely separate improved hybrids, cultivated and wild species. Some diploids grouped according to their geographic origins, among which Musa ornata and IAC-1, and Tjau Lagada and Lidi, while in others no relation was established. There was a trend of clustering among related diploids as: SH3263 and 8694-20; 4279-01 and 9179-03; 1304-06 and 5854-03; 86B79-10 and 7341-03; 86B79-12 and 0337-02; and 9194-04 and 4154-08.
Palabras llave : Musa; diploids; genetic diversity; hybrids; molecular markers.