Pesquisa Agropecuária Brasileira
Print version ISSN 0100-204X
SANTOS, Maria do Desterro Mendes; RIBEIRO, Dalva Graciano and TORRES, Antonio Carlos. Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect. Pesq. agropec. bras. [online]. 2008, vol.43, n.9, pp. 1115-1120. ISSN 0100-204X. http://dx.doi.org/10.1590/S0100-204X2008000900003.
The objective of this work was to evaluate the effect of benzylaminopurine (BAP) additions, in combination with naphthaleneacetic acid (NAA), and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1) and two of NAA (0 and 0.1 mg L-1), and four subculturing periods (30, 60, 90 e 120 days). The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest average number of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.
Keywords : Ananas comosus; in vitro culture; micropropagation.