Pesquisa Agropecuária Brasileira
On-line version ISSN 1678-3921
GONZALEZ, Tehuni Orlando; CAMPANHARO, João Carlos and LEMOS, Eliana Gertrudes de Macedo. Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean. Pesq. agropec. bras. [online]. 2008, vol.43, n.9, pp. 1177-1184. ISSN 1678-3921. http://dx.doi.org/10.1590/S0100-204X2008000900012.
This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fixation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the first experiment, the nodulation and nitrogen fixation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes filled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots filled with soil. The native soil population was identified as Rhizobium sp. and was inefficient in nitrogen fixation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fixation capacity and exhibit differential responses when mixed to PRF-81.
Keywords : Phaseolus vulgaris; biological nitrogen fixation; intergenic space; phylogeny; sequencing; soil rhizobia population.