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Pesquisa Agropecuária Brasileira

Print version ISSN 0100-204X


FORNARI, Darci Carlos et al. Viability of dourado embryos cooled in different cryoprotectant solutions. Pesq. agropec. bras. [online]. 2013, vol.48, n.8, pp.1175-1178. ISSN 0100-204X.

The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.

Keywords : Salminus brasiliensis; cryopreservation; fish embryo; embryo preservation; larvae eclosion.

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