Abstract

FACHINELLO, JOSÉ CARLOS. IN VITRO MULTIPLICATION OF PRUNUS ROOTSTOCKS IN DIFFERENT BAP CONCENTRATIONS IN TWO CULTURE MEDIA. Rev. Bras. Frutic. [online]. 2001, vol.23, n.3, pp. 488-492. ISSN 0100-2945.  http://dx.doi.org/10.1590/S0100-29452001000300007.

The purpose of this work was to determine the best BAP concentration and culture media for the in vitro multiplication of Prunus rootstocks starting from nodal segments. The rootstocks G x N₂₂, GF 677, Mr.S 2/5, Marianna and Mirabolano were tested in two culture media (MS and MS ¾) combined with four BAP concentrations (0.1; 0.3; 0.5 and 0.7 mg.L^-1). It was observed that the genotypes had different performance as the two media and BAP concentrations are concerned. BAP provided better results in the MS ¾ for the rootstocks G x N₂₂ and Mr.S 2/5. For 'Marianna', the best results were found in the MS at 0.7 mg.L^-1 BAP. 'Mirabolano', showed better results in the MS ¾ at 0.5 mg.L^-1 BAP and in the two tested media, the shoot length decreased with the increase for BAP. The rootstock G x N₂₂, had the same effect only in the MS medium. The rootstock GF 677 didn't present good results in micropropagation.

Keywords : cytokinin; peach; tissue culture.