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Revista Brasileira de Fruticultura

Print version ISSN 0100-2945On-line version ISSN 1806-9967

Abstract

BIASI, LUIZ ANTONIO; CARVALHO, DAYSE CRISTINA DE; WOLF, GEFERSON DAVI  and  ZANETTE, FLÁVIO. Organogenetic potential in stem and root tissue of japanese persimmon. Rev. Bras. Frutic. [online]. 2002, vol.24, n.1, pp.29-34. ISSN 0100-2945.  http://dx.doi.org/10.1590/S0100-29452002000100007.

With the objective of studying the morfogenesis in stems and root tissue of japanese persimmon, several experiments were conducted in the field and in vitro. The field experiments consisted of a series of works that involved the cutting of the stem and of the root, the layer and the air-layer of the japanese persimmon ' Fuyu'. In the cutting of the stem, the number of leaves, concentrations of IBA, the located and the total etiolation were tested. In the cutting of the root, the diameter and the position of the stake were tested. The layer was tested in the field and in recipients, being combined with the etiolation of the shoots, ring and application of IBA. In the air-layer the ring and the application of IBA were tested, being the air-layers used for another test with IBA. The experiments in vitro were conducted with juvenile plants, being a works accomplished for the organogenesis induction starting from root segments with BAP, TDZ and cinetina (10µM), combined with IAA (0,01µM), and another for the induction of the budding rooting for the permanence culture medium with 25mg.L-1 of IBA and latter transfer for another exempt culture medium of growth regulators and with activated coal. In all the experiments of cutting, layer and air-layer didn't occur the formation of roots. Just in the root cutting there was organogenesis, where the best budding and rooting responses were obtained with the stakes of larger diameter and in the vertical position. The organogenesis was also observed in root segments in vitro, with a great proliferation of callus and buds induced by the use of TDZ plus IAA. Unlike observed in the field, the in vitro budding took root with a rate of 66%, with a medium emission of 4,7 roots per shoot, being a promising technique to the cloning of the japanese persimmon.

Keywords : Diospyros kaki; cuttings; layers; air-layers; etiolation; growth regulators.

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