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Revista Brasileira de Fruticultura

Print version ISSN 0100-2945On-line version ISSN 1806-9967


SA, MARIA EUGÊNIA LISEI DE  and  BRAGA, MARCELO FIDELES. Evaluation of a protocol to obtain micropropagated plantlets of banana CV. Prata-Anã (subgroup AAB). Rev. Bras. Frutic. [online]. 2002, vol.24, n.1, pp.236-239. ISSN 0100-2945.

The purpose of this work was to evaluate a micropropagation system to cultivar Prata-Anã, observing the main factors of efficiency and limitation in the plantlet production process. The explants were established in the medium MS, supplemented with 5mg/L BAP (benzilaminopurine), 30g/L sucrose, 8g/L agar and pH 5.7. The bud multiplication was carried out in the same medium described above and the plantlet rooting phase was done with half concentration of salt and sucrose, without plant growth regulator. Losses by bacterial contamination were higher during the in vitro establishment and by fungus in the last two subcultures. The highest multiplication rates occurred between the fourth and the fifth subcultures and at the end of the process it was observed higher proportion of plantlets between 30 and 60mm. In relation to the efficiency of the number of glasses processed per operator in the laboratory it was low in the establishment, phase first subculture and rooting, when compared with the exponential multiplication phase of the explants. Even though it was noticed losses in the acclimatization phase, it was observed the occurrence of 1% of plantlets with morphological variation.

Keywords : micropropagation; Musa sp..

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