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Revista Brasileira de Fruticultura

Print version ISSN 0100-2945On-line version ISSN 1806-9967


RODRIGUES, Alexandre Couto et al. Establishment and multiplication in vitro of Prunus sp. in different culture media. Rev. Bras. Frutic. [online]. 2003, vol.25, n.1, pp.131-133. ISSN 0100-2945.

The work was performed aiming to evaluate different media for the establishment and in vitro multiplication of some rootstocks species of Prunus. Microcutings of 1,0cm long were incubated under 20 mE.m-2.s-1 radiation provided by white fluorescent lamps, 16-hour photoperiod and temperature of 25±2ºC. The establishment media used were as follow: MS, ¾ MS, SH and Villegas, and the multiplication media used were: SH and ¾ MS. The ¾ MS medium was also tested with different agar concentrations (4.5; 5.5; 6.5 g.L-1). The percentage of establishment, contamination, oxidation and growing of the explants were evaluated. The Villegas medium provided low oxidation during the period of in vitro establishment. The ¾ MS medium provided higher percentage of establishment of explants. In the multiplication phase, the highest growth percentage, multiplication rate and bud number, were found in ¾ MS medium with 5.5 g.L-1 agar.

Keywords : peach; plum; micropropagation.

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