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Revista Brasileira de Fruticultura
On-line version ISSN 1806-9967
CANTAGALLO, Fabrizzio de Souza et al. Micropropagation of 'Swingle' citrumelo through axilary buds in vitro culture. Rev. Bras. Frutic. [online]. 2005, vol.27, n.1, pp. 136-138. ISSN 1806-9967. http://dx.doi.org/10.1590/S0100-29452005000100036.
The use of micropropagation to nursery tree production is considered an important technique of propagation, although it is not usual in citrus industry, where rootstocks are produced by seedlings. However, it might be a viable alternative when the demand for nursery material is higher than seed availability. This work aimed to micropropagate 'Swingle' citrumelo through axilary buds, excised from in vitro seedlings cultivated in MT medium, six weeks after seed incubation, and submitted to combinations of BAP - 6-benzilaminapurine (0,0; 0,5; 1,0 e 1,5 mg L-1 ) and NAA - naftalene acetic acid (0,0 e 0,5 mg L-1). Shoots were rooted in RMAN medium and then acclimated. The number of induced shoots was affected by concentrations of BAP. The best vegetative development of the acclimated plants was accomplished in shoots from treatments with lower growth regulator concentrations.
Keywords : citrus; rootstock; shoot multiplication; tissue culture.