Revista Brasileira de Fruticultura
Print version ISSN 0100-2945
SANTOS, Breno Régis et al. Micropropagation of "pequizeiro" (Caryocar brasiliense Camb.). Rev. Bras. Frutic. [online]. 2006, vol.28, n.2, pp. 293-296. ISSN 0100-2945. http://dx.doi.org/10.1590/S0100-29452006000200031.
Due the presence of seed dormancy, the propagation of Caryocar brasiliense requires studies in order to obtain young asexually plants. This work had as objective contribute to the establishment of a "pequizeiro" micropropagation protocol. For shoot induction, explants were inoculated in WPM medium with different concentrations and interactions of BAP and NAA. For rooting induction, shoots obtained in vitro were transferred to WPM medium supplemented with different concentrations of IBA in the presence or absence of activated charcoal. The best treatment for shoot induction was the use of 0,05mg L-1 ANA + 0,75mg L-1 BAP, which produced an average of 6 shoots/explant (17,4) providing the highest rate of multiplication (8,7). The use of 3mg L-1 IBA promoted root induction in all explants generating average of 12,87 roots per explant. Roots developed in the presence of activated charcoal presenting higher length (33,16 mm) and higher number of secondary roots (19,53).
Keywords : 6-benzylaminopurine; organogenesis; indolbutiric acid; acclimatization; phytorregulator; culture media.