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Revista Brasileira de Fruticultura
Print version ISSN 0100-2945
SCHINOR, Evandro Henrique et al. Citrus sp. organogenesis in vitro from different epicotyl's regions. Rev. Bras. Frutic. [online]. 2006, vol.28, n.3, pp. 463-466. ISSN 0100-2945. http://dx.doi.org/10.1590/S0100-29452006000300027.
The establishment of an in vitro plant regeneration protocol is essential for the use of genetic transformation techniques in Citrus breeding programs. Aiming to obtain an efficient protocol of in vitro regeneration for sour orange (Citrus aurantium), sweet oranges 'Natal' and 'Pêra' (C. sinensis), 'Volkamer' lemon (C. volkameriana) and 'Carrizo' citrange (Poncirus trifoliata x C. sinensis), the morphogenetic response was evaluated for different epicotyl's regions (basal, medium and apical) regarding the distance from the cotyledonary node, in presence (1,0 mg/L-1) or absence of 6-BAP in MT medium. After 60 days, the percentage of responsive explants and number of shoots per explants were evaluated. The morphogenetic response related to the epicotyl's region and the presence or absence of the cytokinin (6-BAP) were genotype influenced. The presence of 6-BAP in the culture medium showed a greater percentage of responsive explants for 'Carrizo' citrange. The addition of 6-BAP to the culture medium provided an increase in the number of shoots per explants for 'Carrizo' citrange, 'Natal' sweet orange and 'Volkamer' lemon.
Keywords : adventitious shoots; tissue culture; morphogenesis.