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Revista Brasileira de Fruticultura

Print version ISSN 0100-2945On-line version ISSN 1806-9967


CARVALHO, Dayse Cristina de; MOURAO FILHO, Francisco de Assis Alves; MENDES, Beatriz Madalena Januzzi  and  CARVALHO, Carlos Roberto de. Plant regeneration after protoplast fusion of 'Page' tangelo and 'Lau Tau' pummelo. Rev. Bras. Frutic. [online]. 2007, vol.29, n.2, pp.329-332. ISSN 0100-2945.

This work aimed to produce somatic hybrid between 'Page' tangelo and 'Lau Tau' pummelo in an attempt to regenerate a similar rootstock to sour orange, because this species is considered a probable hybrid between C. reticulata and C. grandis. After protoplast isolation, fusion and culture regenerated shoots were in vitro grafted on 'Hamlin' sweet orange. Seventeen plants were acclimatized in a greenhouse. Citometric flow analyses revealed that all plants are diploid. RAPD molecular markers of regenerated plants had the same pattern as compared to 'Page' tangelo. However, all plants had phenotypic traits different from both genitors.

Keywords : citrus; genetic improvement; rootstock; somatic hybridization; tissue culture.

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