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Revista Brasileira de Fruticultura
On-line version ISSN 1806-9967
WICKERT, Ester et al. Phylogenetic relationships and diversity of Guignardia spp isolated from different hosts on ITS1-5,8S-ITS2 region . Rev. Bras. Frutic. [online]. 2009, vol.31, n.2, pp. 360-380. ISSN 1806-9967. http://dx.doi.org/10.1590/S0100-29452009000200010.
Fungi of Guignardia genera are commonly isolated from different plant species and most of the time they are characterized as endophytics. However, some species of this genus, as G. citricarpa and G. psidii are known as causal agents of serious diseases that affect cultures, such as the Citrus Black Spot and the guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other cultures. This work has the objective of isolate, identify and characterize the genetic diversity present among Guignardia isolates obtained from citrus, mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry by analysis of DNA sequence from cístron ITS1-5,8SITS2. It was verified that the obtained isolates belong to G. mangiferae and G. citricarpa species. Two different Guignardia types, that were found in mango, could not be identified in species, and do not belong to none of the species deposited in GenBank. So, this work found that mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry host only G. mangiferae, whereas citrus hosts G. mangiferae and G. citricarpa species. Mango hosts three different Guignardia, G. mangiferae and two others types that remain without identification of the species level. It was also verified that isolates of Guignardia obtained from guava fruit rot symptoms were identified as G. mangiferae.
Keywords : endophytic fungi; pathogenic; genetic variability.