Revista Brasileira de Fruticultura
Print version ISSN 0100-2945
AYUB, Ricardo Antonio; SPINARDI, Bruna; BASSO, Marcos Fernando and BIASI, Luiz Antonio. Induction of multibrotacion in vitro in videira cv. Bordô. Rev. Bras. Frutic. [online]. 2010, vol.32, n.3, pp. 675-681. Epub Sep 24, 2010. ISSN 0100-2945. http://dx.doi.org/10.1590/S0100-29452010005000091.
The Micropropagation allows mass propagation and may contribute to meet the demands of mother plants and seedlings of proven genetic quality and health of the vine. Different culture media plus or absence of plant growth regulators has been used frequently to assist in the induction of shoots. The study aimed to evaluate the means of culture and GZ and MS full or half the concentration of salts plus BAP for induction of multiple on the grapevine cv. Bordo. The experiment was conducted in a randomized design in a factorial 4 x 4, with four culture media (MS, MS/2, GZ, GZ/2) and four concentrations of BAP (0, 2.5, 5 and 10 μM) with four replications. It was evaluated at 30 days the number of micro-shoots per cutting, number of new micro-cuttings per shoot, number of leaves per shoot, mean length of shoots, number of roots by micro-cutting and length of the main root. The culture medium for more efficient induction of multiple in grapevine cv. Bordo and the development of the shoot is the MS medium plus 2.5 μM of BAP.
Keywords : Vitis labrusca; cytokinin; micropropagation.