Revista Brasileira de Fruticultura
Print version ISSN 0100-2945
MOTA, Monalize Salete et al. Molecular identification of S-alleles of gametophytic incompatibility in Prunus salicina Lindl.. Rev. Bras. Frutic. [online]. 2010, vol.32, n.3, pp. 798-807. Epub Sep 24, 2010. ISSN 0100-2945. http://dx.doi.org/10.1590/S0100-29452010005000096.
Commercial plum orchards must contain at least two cultivars, in order to ensure good fruit set and thus high economic yields, because its carries the gametophytic incompatibility system, that inhibits the selfing in a great number of cultivares. The aim of this work was to identify by PCR (Polymerase Chain Reaction) the S-alleles related to gametophytic incompatibility in eleven Prunus salicina (Lindl.) cultivars. The Santa Rosa, Santa Rita, Reubennel, Pluma 7, América, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaíba) e Harry Pickstone cultivars were analysed using three pairs of specific primers for amplifying the S-alleles. The PCR conditions and primers set allowed a characterization of S-alleles in the japonese plum and the identification of the most compatible pollinators to some commercial cultivars. Sequencing analysis of some amplified S-alleles showed high similarity to nucleotides sequences already identified in other studies with Prunus spp. However, complete sequences of a large number of S-alleles it is necessary to get a more precisely identity of relationship among this S-alleles.
Keywords : Japonese plum; gametophytic self-incompatibility; PCR; S-alleles.