SciELO - Scientific Electronic Library Online

 
vol.39 número4MURCHA-DE-CERATOCYSTIS NAS MANGUEIRAS ‘UBÁ’ E ‘DURA’ SUBMETIDAS A DÉFICIT HÍDRICOMOSCAS FRUGÍVORAS E SEUS PARASITOIDES NO POLO DE FRUTICULTURA DE LIVRAMENTO DE NOSSA SENHORA, BAHIA,COM REGISTRO DE INTERAÇÕES INÉDITAS índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Revista Brasileira de Fruticultura

versão impressa ISSN 0100-2945versão On-line ISSN 1806-9967

Resumo

FAGANELLO, FERNANDA DE SILLOS et al. MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE. Rev. Bras. Frutic. [online]. 2017, vol.39, n.4, e-518.  Epub 09-Out-2017. ISSN 0100-2945.  http://dx.doi.org/10.1590/0100-29452017518.

Citrus black spot, a fungal disease caused by the quarantine fungus Guignardia citricarpa, restricts the exportation of fresh fruit to countries in the European Union. The occurrence of latent infections and the time required for diagnosis using conventional methods have brought about the need to validate fast, efficient and reproducible molecular techniques to detect the pathogen in asymptomatic tissue. As such, this study aims to detect G. citricarpa in the symptomatic fruit and asymptomatic leaf tissue of sweet oranges by conventional and real-time polymerase chain reaction (PCR). Specificity and limit of detection (LOD) were assessed in tissue samples of fruit lesions and asymptomatic leaves. Low concentrations of the fungus were found in asymptomatic leaves. Under these conditions, real-time PCR proved to be viable, reproducible and highly sensitive to detection of the pathogen.

Palavras-chave : Citriculture; molecular diagnosis; Phyllosticta citricarpa.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf )