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vol.40 número1AVALIAÇÃO DA ATIVIDADE HEMOAGLUTINANTE E INIBIDORA DE ENZIMAS DIGESTÓRIAS DE EXTRATOS OBTIDOS DE DIFERENTES PARTES DA ATEMOIAETHEPHON E CLORETO DE CÁLCIO, UMA COMBINAÇÃO QUE PROMOVE A MELHORIA DA COR DE UVAS ‘RUBI’ índice de autoresíndice de assuntospesquisa de artigos
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Revista Brasileira de Fruticultura

versão impressa ISSN 0100-2945versão On-line ISSN 1806-9967

Resumo

SANCHEZ-GONZALEZ, Enrique Ignacio et al. DIFERENTIAL EXPRESSED SEQUENCE TAGS OF MEXICAN RACE AVOCADO FRUITS (Persea americana Mill. var. drymifolia). Rev. Bras. Frutic. [online]. 2018, vol.40, n.1, e-184.  Epub 15-Fev-2018. ISSN 0100-2945.  http://dx.doi.org/10.1590/0100-29452018184.

In this study, Differential Expressed Sequence Tags (ESTs) of immature fruits of Persea americana Mill var. drymifolia of the state of Nuevo León, Mexico were developed and identified. Ten genotypes with fruits of different size and shape were selected to generate ESTs by the differential display technique. In total, 393 amplified differential fragments were obtained, 82 fragments were sequenced and edited for identification and comparison with NCBI nucleotide and protein databases. Forty sequences showed significant similarity to mRNA sequences and / or sequences of hypothetical or predicted proteins belonging to P. americana and / or other genera. Some sequences were related to enzymes such as flavanone-3-hydroxylase (F3H), lecithin-cholesterol acyltransferase, glutathione-S-transferase microsomal and pleiotropic drug resistance protein. With the information of the nucleotide composition of ESTs, primers could be designed to quantify expression levels by real-time RT-PCR of genes in different phenological stages of the fruit and to make comparisons among genotypes that allow determining alternative uses of their fruits.

Palavras-chave : avocado; Mexican race; differential display; EST.

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