SciELO - Scientific Electronic Library Online

 
vol.40 número1Índices paramétricos e não paramétricos aplicados na seleção de progênies de maracujazeiro-azedoCaracterização física, química, microbiológica e sensorial de geleias de Manipuçá para a merenda escolar municipal de Fortaleza-CE índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Revista Brasileira de Fruticultura

versão impressa ISSN 0100-2945versão On-line ISSN 1806-9967

Resumo

SACOMAN, NAYARA NIELE et al. Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea. Rev. Bras. Frutic. [online]. 2018, vol.40, n.1, e-607.  Epub 26-Fev-2018. ISSN 0100-2945.  http://dx.doi.org/10.1590/0100-29452017607.

Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.

Palavras-chave : PTA-ELISA; fruit hardening virus; passion fruit; plant resistance.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf epdf )