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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064

Abstract

QUEIROZ, Sonia C. N.; COLLINS, Carol H.  and  JARDIM, Isabel C. S. F.. Methods of extraction and/or concentration of compounds found in biological fluids for subsequent chromatographic determination. Quím. Nova [online]. 2001, vol.24, n.1, pp.68-76. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422001000100013.

When organic compounds present in biological fluids are analysed by chromatographic methods, it is generally necessary to carry out a prior sample preparation due the high complexity of this type of sample, especially when the compounds to be determinated are found in very low concentrations. This article describes some of the principal methods for sample preparation in analyses of substances present in biological fluids. The methods include liquid-liquid extraction, solid phase extraction, supercritical fluid extraction and extraction using solid and liquid membranes. The advantages and disadvantages of these methods are discussed.

Keywords : sample preparation; biological fluids; extraction methods.

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