SciELO - Scientific Electronic Library Online

 
vol.24 issue2Flow injection spectrophotometric system for hydrogen peroxide monitoring in photo-Fenton degradation processesComparative study of analytical methods for iron and titanium determination in Portland cement author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064

Abstract

LUCA, Gilmara C.  and  REIS, Boaventura F.. Flow system for spectrophotometric urea determination in animal blood plasma employing leguminous plant as natural urease enzyme source. Quím. Nova [online]. 2001, vol.24, n.2, pp.191-194. ISSN 0100-4042.  https://doi.org/10.1590/S0100-40422001000200008.

A spectrophotometric flow injection analysis (FIA) procedure employing natural urease enzyme source for the determination of urea in animal blood plasma was developed. Among leguminous plants used in the Brazilian agriculture, the Cajanus cajan specie was selected as urease source considering its efficiency and availability. A minicolumn was filled with leguminous fragments and coupled to the FIA manifold, where urea was on-line converted to ammonium ions and subsequently it was quantified by spectrophotometry. The system was employed to determine urea in animal plasma samples without any prior treatment. Accuracy was assessed by comparison results with those obtained employing the official procedure and no significant difference at 90 % confidence level was observed. Other profitable features such as an analytical throughput of 30 determinations per hour, a reagent consumption of 19.2 mg sodium salicylate, 0.5 mg sodium hipochloride and a relative standard deviation of 1.4 % (n= 12) were also obtained.

Keywords : urea determination; leguminous plant urease source; flow analysis; animal blood plasma.

        · text in Portuguese     · Portuguese ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License