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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


RIOS, Magda Dias Gonçalves  and  PENTEADO, Marilene De Vuono Camargo. Determination of a-Tocopherol (vitamin E) in irradiated garlic by high performance liquid chromatography (HPLC). Quím. Nova [online]. 2003, vol.26, n.1, pp.10-12. ISSN 0100-4042.

The effects of 60Co ionizing radiations in doses of 0, 75, 100, 150, 200 and 250Gy on garlic, upon the a-tocopherol concentration were studied. The a-tocopherol contents were established by high performance liquid chromatography (HPLC), after direct hexane extraction from the garlic samples. The a-tocopherol was determined through normal-phase column, and mobile phase was composed by hexane: iso-propyl alcohol (99:01 v/v), with 2mL/min flow rate and fluorescence detector. It is statistically shown that an irradiation dose of up to 150 Gy does not affect the garlic a-tocopherol content.

Keywords : garlic; a-tocopherol; HPLC.

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