Print version ISSN 0100-4042
MARTINS JUNIOR, Helio Alves et al. Determination of chloramphenicol residues in industrialized milk and honey samples using LC-MS/MS. Quím. Nova [online]. 2006, vol.29, n.3, pp. 586-592. ISSN 0100-4042. http://dx.doi.org/10.1590/S0100-40422006000300030.
The present work shows a method for the determination of chloramphenicol (CAP) antibiotic in milk, powder milk and honey. The solid phase extraction and liquid-liquid extraction were applied as a clean-up and pre-concentration strategies followed by LC-ESI/MS/MS analysis. The recovery was studied for different fortification levels from 0.05 to 1.00 µg L-1 in milk, showing values between 91 101% and RSD bellow 8.0%, while honey was spiked with a concentration of 0.20 µg kg-1 yelding a mean recovery of 83% and RSD of 6.5%. The quantification transition 321>152 showed a LOD of 0.52 ng kg-1 and LOQ of 1.85 ng kg-1.
Keywords : chloramphenicol; milk; honey.