SciELO - Scientific Electronic Library Online

 
vol.29 número6Metodologia para análise simultânea de ácido nicotínico, trigonelina, ácido clorogênico e cafeína em café torrado por cromatografia líquida de alta eficiência índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

Compartilhar


Química Nova

versão impressa ISSN 0100-4042

Resumo

SILVA, Gisele Rodrigues da et al. Determination of lamivudine, stavudine and nevirapine in tablets by high performance liquid chromatography. Quím. Nova [online]. 2006, vol.29, n.6, pp. 1159-1163. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422006000600002.

A high performance liquid chromatography method was developed to quantify lamivudine, stavudine and nevirapine combined in tablets. The separation was carried out in less than 10 min using a phosphate buffer of pH 3.0 and acetonitrile (75:25, v/v) as mobile phase, a LiChrospher ODS column and UV detection at 266 nm. The method was linear over the range of 15-135 µg/mL (lamivudine), 4-36 µg/mL (stavudine) and 20-180 µg/mL (nevirapine). The accuracy ranged from 98.56 to 102.04% and intra-day and inter-day precision was less than 1% for the three drugs. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.

Palavras-chave : antiretroviral; HPLC; validation.

        · texto em Português     · pdf em Portuguêspdf em Português