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versão On-line ISSN 1678-7064
ALVES, Sandriel Trindade; DIAS, Rafael Carlos Eloy; BENASSI, Marta de Toledo e SCHOLZ, Maria Brígida dos Santos. HPLC analysis of nicotinic acid, trigonelline, chlorogenic acid and caffeine in roasted coffee. Quím. Nova [online]. 2006, vol.29, n.6, pp. 1164-1168. ISSN 1678-7064. http://dx.doi.org/10.1590/S0100-40422006000600003.
A reverse phase liquid chromatography method was developed for simultaneous determination of trigonelline, caffeine, nicotinic and chlorogenic (5-CQA) acids in roasted coffee. A gradient of acetic acid/acetonitrile was used as mobile phase and detection was carried out in the UV. The samples were extracted with acetonitrile/water (5:95 v/v) at 80 ºC/10 min. Good recovery (89 to 104%), repeatability and linearity were obtained. Detection limits of 0.01, 0.15, 0.04 and 0.04 mg mL-1 were observed for nicotinic acid, trigonelline, 5-CQA and caffeine. The method, applied to arabica and robusta coffees with different degrees of roasting, was efficient and fast (~35 min) and also allowed identification of cinnamic acids.
Palavras-chave : Coffea arabica; Coffea canephora; 5-CQA.