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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


PAULA, Ariela V. et al. Comparative performance of Candida rugosa lipase immobilized on polysiloxane polyvinyl alcohol hybrid support using different methodologies. Quím. Nova [online]. 2008, vol.31, n.1, pp.35-40. ISSN 0100-4042.

The efficiency for immobilizing microbial Candida rugosa lipase on a hybrid matrix of polysiloxane polyvinyl alcohol, by adsorption, covalent coupling and encapsulation was compared. The activities of immobilized derivatives were evaluated using p-nitrophenylpalmitate (hydrolysis) and butyric acid and butanol (esterification) as substrates. Operational stability and storage tests were also performed. Among the procedures tested, the proposed matrix was efficient for immobilizing C. rugosa lipase by adsorption and covalent coupling techniques and unsuitable for encapsulation purposes. The results reveal that better catalytic properties in both aqueous and organic media were demonstrated by the covalent coupling POS-PVA immobilized lipase, including also satisfactory half-life and good storage stability.

Keywords : lipase; hybrid support; adsorption.

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