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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


SOARES, Fernanda Barros; SOUSA, Juliana Morais de  and  DIMENSTEIN, Roberto. Evaluation of the retinol concentration in UHT milk commercialized in Natal, RN. Quím. Nova [online]. 2008, vol.31, n.2, pp.268-269. ISSN 0100-4042.

Deficiency of micronutrients is a public health problem. Cow milk is a source of retinol. The objective of this study is to evaluate the retinol concentration in milk commercialized in Natal/RN. Ten samples were taken of each brand of UHT milk. Vitamin content was determined by HPLC using the Shimadzu LC-10 AD Chromatograph, coupled to the Shimadzu SPD 10 A UV-VIS Detector and the Shimadzu C-R6A Chromatopac Integrator with Shim-pack CLC-ODS (M) column, measuring 4.6 mm x 25 cm. The mobile phase was 100% methanol, with a flow of 1 mL/min. The mean retinol concentration varied between 22.7 ± 4.9 µg/100 mL and 44.1 ± 4.1 µg/100 mL, with the differences statistically significant (p<0.001). Only one of the 7 brands had retinol concentration below the normal requirements for human consumption.

Keywords : milk; cow; retinol.

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