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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064

Abstract

GARDA, Jaqueline  and  BADIALE-FURLONG, Eliana. Optimization of the methodology for deoxynivalenol derivatization by experimental planning. Quím. Nova [online]. 2008, vol.31, n.2, pp.270-274. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422008000200015.

The objective of this work was to optimize the derivatization reaction for determining deoxynivalenol (DON) by gas chromatography employing an experimental planning procedure. The factors were: temperature, reaction time, catalyst and trifluoroacetic anhydride concentration. The relative peak areas were used to evaluated the effects. The best conditions for DON derivatization were 200 µL TFAA and 18 mg sodium bicarbonate for 6 min at 74 ºC for 7 to 21 µg of DON. Under these conditions, the detection limit was 1.4 µg of DON.

Keywords : deoxynivalenol; derivatization; methodology.

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