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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


GROTTO, Denise et al. Evaluation of the stability of the oxidative stress plasmatic biomarker: malondialdehyde. Quím. Nova [online]. 2008, vol.31, n.2, pp.275-279. ISSN 0100-4042.

Malondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at -20 ºC, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at -20 ºC.

Keywords : oxidative stress; malondialdehyde; stability.

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