SciELO - Scientific Electronic Library Online

 
vol.31 issue3Laboratory scale assessment of an electrolytic process for water treatmentMagnetic niobia as adsorbent of organic contaminants in aqueous medium: effect of temperature and pH author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064

Abstract

PRADO, Guilherme et al. Determination of aflatoxin B1 (Piper nigrum L.) and oregano (Origanum vulgare L.) by thin-layer chromatography and densitometry. Quím. Nova [online]. 2008, vol.31, n.3, pp.514-517. ISSN 0100-4042.  https://doi.org/10.1590/S0100-40422008000300009.

An analytical study based on extraction with methanol-water, immunoaffinity cleanup and separation, identification and quantification of aflatoxin B1 by thin-layer chromatography,in ground black and white pepper and oregano was carried out. Validation of the applied methodology was done through accuracy and precision studies. Recoveries of aflatoxin B1 and relative standard deviations, from spice samples spiked at levels from 4.86 to 97.70 µg/kg, were, respectively, higher than 72% and lower than 20%. Application to spice samples available in Minas Gerais state, purchased at popular markets, showed no contamination with aflatoxin B1.

Keywords : aflatoxin B1; methodology; spices.

        · text in Portuguese     · Portuguese ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License