SciELO - Scientific Electronic Library Online

 
vol.31 issue3Ring-closing olefin metathesis: a powerful tool for the synthesis of natural macrocyclesEvaluation of chemical precipitation and encapsulation techniques in the treatment and joint disposal of liquid wastes containing chromium and laboratory glassware author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064

Abstract

CANTU, Marcelo Delmar; CARRILHO, Emanuel; WULFF, Nelson Arno  and  PALMA, Mario Sérgio. Peptide sequencing using mass spectrometry: a practical guide. Quím. Nova [online]. 2008, vol.31, n.3, pp.669-675. ISSN 0100-4042.  https://doi.org/10.1590/S0100-40422008000300034.

This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.

Keywords : proteomic analysis; mass spectrometry; peptide sequencing.

        · text in Portuguese     · Portuguese ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License