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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


CANTU, Marcelo Delmar; CARRILHO, Emanuel; WULFF, Nelson Arno  and  PALMA, Mario Sérgio. Peptide sequencing using mass spectrometry: a practical guide. Quím. Nova [online]. 2008, vol.31, n.3, pp.669-675. ISSN 0100-4042.

This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.

Keywords : proteomic analysis; mass spectrometry; peptide sequencing.

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