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Química Nova

Print version ISSN 0100-4042On-line version ISSN 1678-7064


LIMA, Kássio M. G.; TREVISAN, Marcello G.; POPPI, Ronei J.  and  ANDRADE, João Carlos de. Non-destructive determination of total nitrogen in plants by diffuse reflectance near infrared spectroscopy. Quím. Nova [online]. 2008, vol.31, n.3, pp.700-703. ISSN 0100-4042.

Diffuse reflectance near-infrared (DR-NIR) spectroscopy associated with partial least squares (PLS) multivariate calibration is proposed for a direct, non-destructive, determination of total nitrogen in wheat leaves. The procedure was developed for an Analytical Instrumental Analysis course, carried out at the Institute of Chemistry of the State University of Campinas. The DR-NIR results are in good agreement with those obtained by the Kjeldhal standard procedure, with a relative error of less than ± 3% and the method may be used for teaching purposes as well as for routine analysis.

Keywords : total nitrogen; near-infrared diffuse reflectance; teaching activity.

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