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On-line version ISSN 1678-7064
HOFFMANN-RIBANI, Rosemary and RODRIGUEZ-AMAYA, Delia B.. Optimization of a method for determination of flavonols and flavones in fruits by HPLC using statistical design and response surface analysis. Quím. Nova [online]. 2008, vol.31, n.6, pp. 1378-1384. ISSN 1678-7064. http://dx.doi.org/10.1590/S0100-40422008000600020.
This work optimized the HPLC conditions for the simultaneous determination of luteolin, apigenin, myricetin, quercetin and kaempferol in aglycone form, as well defined the best conditions for hydrolysis/extraction of these flavonoids in fruits, using the statistical central composite design and response surface analysis. A reverse phase method was developed using a gradient of methanol/water acidified with 0.3% formic acid as mobile phase and a photodiode array detector. The samples were extracted with methanol/water (50:50 v/v) at 90 ºC. The optimum time and HCl concentration varied for the different fruits investigated, demonstrating the necessity of optimizing these conditions for each fruit analyzed. Good recovery (87.1 to 96.3%), repeatability and linearity were obtained.
Keywords : flavonoids; HPLC analysis; statistical central composite design.