Abstract

MERTINS, Omar et al. Characterization of soybean phosphatidylcholine purity by ¹H and ³¹P NMR. Quím. Nova [online]. 2008, vol.31, n.7, pp. 1856-1859. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422008000700043.

A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of ¹H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68±1.37 was determined. Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. ³¹P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.

Keywords : NMR; phosphatidylcholine; purity determination.