On-line version ISSN 1678-7064
CASAGRANDE, Rúbia et al. Method validation and stability study of quercetin in topical emulsions. Quím. Nova [online]. 2009, vol.32, n.7, pp. 1939-1942. ISSN 1678-7064. http://dx.doi.org/10.1590/S0100-40422009000700041.
This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.
Keywords : quercetin; HPLC; validation.