Resumo

ANTUNES, Marina Venzon; FELTRACO, Lilian de Lima; MORISSO, Fernando Dal Pont  e  LINDEN, Rafael. Determination of 2,5-hexanedione in urine by high performance liquid chromatography after derivatization with 2,4-dinitrophenilhidrazine. Quím. Nova [online]. 2011, vol.34, n.1, pp. 151-155. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422011000100028.

A method for quantifying urinary 2,5-hexanedione was optimized and validated. Urine samples were hydrolyzed and derivatized with 2,4-dinitrophenylhydrazine. The analyte was separated in a high performance liquid chromatography system with a diode array detector, using a C18 column (150 x 4.6 mm, p.d. 5 µm) and a mobile phase composed of phosphate buffer pH 2.3:acetonitrile (40:60, v/v), at a flow rate of 1 mL/min. The chromatograms were monitored at 334 nm. Retention time was 7.3 minutes. Main validation parameters were: coefficient of determination: 0.9994, accuracy: 96 to 107%; intra-assay precision (RSD): 3.08 to 6.72%; inter-assay precision (RSD): 2.54 to 8.17% and limit of quantitation of 0.19 µg/mL.

Palavras-chave : 2,5-hexanedione; HPLC; 2,4-dinitrophenylhydrazine.