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Química Nova

Print version ISSN 0100-4042

Abstract

MAROTTA-OLIVEIRA, Samantha Sant'Anna  and  MARCHETTI, Juliana Maldonado. Determinação de heparina fracionada em preparações farmacêuticas utilizando turbidimetria. Quím. Nova [online]. 2011, vol.34, n.6, pp.1063-1067. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422011000600025.

A turbidimetric method has been used for quantification of fractionated heparin (FH) in pharmaceutical dosage. The UV detection at two wavelengths (290 and 500 nm) showed a significant increase in sensitivity of the method, specificity, and linearity to range 5.0-50.0 µg mL-1 and 50.0-200.0 µg mL-1, respectively (r < 0.99). At both wavelengths, the method was precise (inter-assay CV < 5.0%, and intra-assay CV < 3.0%), accurate (maximum deviation of ± 12%), and robust to the parameters evaluated. Turbidimetry proved to be easy, inexpensive and relatively fast. The results obtained attest to the reliability of the method.

Keywords : fractionated heparin; quantitative analysis; turbidimetry.

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