versão impressa ISSN 0100-4042
BAIERLE, Marília et al. Serum quantification of vitamin C by HPLC-UV and stability study. Quím. Nova [online]. 2012, vol.35, n.2, pp. 403-407. ISSN 0100-4042. http://dx.doi.org/10.1590/S0100-40422012000200030.
Vitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 °C, in serum and supernatant were also evaluated. Analysis showed r2 > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at -20 and -80 °C or in supernatant at -80 °C the vitamin C levels remain stable for 30 and 12 days, respectively.
Palavras-chave : HPLC; vitamin C; stability.