SciELO - Scientific Electronic Library Online

 
vol.35 issue3Using the freshwater bivalve Anodontites tenebricosus (LEA, 1834) as a biomonitor of metals in the Ribeira de Iguape River2D-QSAR studies based on topological descriptors and molecular fragments for a series of azole derivatives active against Candida albicans author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Química Nova

Print version ISSN 0100-4042

Abstract

BEZERRA, Daniel P. et al. Sensitive method for determination of piplartine, an alkaloid amide from Piper species, in rat plasma samples by liquid chromatography-tandem mass spectrometry. Quím. Nova [online]. 2012, vol.35, n.3, pp. 460-465. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422012000300004.

Piplartine (PPTN) is an alkaloid amide found in Piper species that presents different activities. PPTN determination in rat plasma is necessary to better understand its biological effects. The aim of this study was to develop a sensitive LC-MS/MS method for the determination of PPTN in rat plasma. The performance criteria for linearity, sensitivity, precision, accuracy, recovery, and stability have been assessed and were within the recommended guidelines. The validated method proved to be suitable in a pilot study of PPTN kinetic disposition in rat plasma after a single intraperitoneal dose, and represents an appropriate tool to further pharmacokinetic studies.

Keywords : piplartine; LC-MS [MS]; rat plasma analysis.

        · text in English     · pdf in English