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versão impressa ISSN 0100-4042
MARQUES, Graziella Silvestre et al. Evaluation of procedure for spectrophotometric quantification of total flavonoids in leaves of Bauhinia forficata Link. Quím. Nova [online]. 2012, vol.35, n.3, pp.517-522. ISSN 0100-4042. http://dx.doi.org/10.1590/S0100-40422012000300014.
Interest in analytical methods for quality control of herbal drugs has grown sharply due to the scarcity of monographs in official manuals. Thus, the aim of the present study was to evaluate analytical procedures for quantitative determination of flavonoids from leaves of Bauhinia forficata Link (pata-de-vaca). Two procedures for quantification of total flavonoids (with and without acid hydrolysis) by spectrophotometry were tested. The proposed methods proved to be specific, sensitive, precise, accurate and robust, being suitable for routine laboratory use.
Palavras-chave : Bauhinia forficata Link; flavonoids; UV-Vis spectrophotometric.