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Química Nova

Print version ISSN 0100-4042


KIRSCH, Larissa de Souza et al. Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System. Quím. Nova [online]. 2012, vol.35, n.10, pp.1912-1915. ISSN 0100-4042.

A full two-level factorial design was employed to study the influence of PEG molar mass (MMPEG), PEG concentration (CPEG) and phosphate concentration (CPHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MMPEG = 6000 g mol-1, CPEG = 17.5% (w/w) and CPHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.

Keywords : Lentinus citrinus; proteases; aqueous two-phase systems.

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