SciELO - Scientific Electronic Library Online

vol.36 issue2In vitro activity of essential oils of Lippia sidoides and Lippia gracilis and their major chemical components against Thielaviopsis paradoxa, causal agent of stem bleeding in coconut palmsTreated domestic sewage: kinetics of Escherichia coli and total coliform inactivation by oxidation with hydrogen peroxide author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Química Nova

Print version ISSN 0100-4042


MENDES, Adriano A.; CASTRO, Heizir F. de  and  GIORDANO, Raquel de L. C.. Screening of organic supports and activation protocols for immobilization and stabilization of lipase from Thermomyces lanuginosus. Quím. Nova [online]. 2013, vol.36, n.2, pp.245-251. ISSN 0100-4042.

Lipase from Thermomyces lanuginosus was covalently immobilized on activated poly-hydroxybutyrate, sugarcane bagasse and the chemically modified hybrid hydrogel chitosan-alginate prepared by different strategies. Among the tested supports, chitosan-alginate chemically modified with 2,4,6-trinitrobenzenesulfonic acid rendered derivatives with the highest hydrolytic activity and thermal-stability, 45-fold more stable than soluble lipase and was then selected for further studies. The pH of maximum activity was similar for both immobilized and free lipase (pH 8.0) while optimum temperature was 5 - 10 ºC higher for the immobilized lipase. Higher yields in the butyl butyrate synthesis were found for the derivatives prepared by activation with glycidol and epichlorohydrin.

Keywords : covalent attachment; lipase; organic supports.

        · text in Portuguese     · Portuguese ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License