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Química Nova

Print version ISSN 0100-4042

Abstract

MENDES, Adriano A.; CASTRO, Heizir F. de  and  GIORDANO, Raquel de L. C.. Screening of organic supports and activation protocols for immobilization and stabilization of lipase from Thermomyces lanuginosus. Quím. Nova [online]. 2013, vol.36, n.2, pp.245-251. ISSN 0100-4042.  https://doi.org/10.1590/S0100-40422013000200008.

Lipase from Thermomyces lanuginosus was covalently immobilized on activated poly-hydroxybutyrate, sugarcane bagasse and the chemically modified hybrid hydrogel chitosan-alginate prepared by different strategies. Among the tested supports, chitosan-alginate chemically modified with 2,4,6-trinitrobenzenesulfonic acid rendered derivatives with the highest hydrolytic activity and thermal-stability, 45-fold more stable than soluble lipase and was then selected for further studies. The pH of maximum activity was similar for both immobilized and free lipase (pH 8.0) while optimum temperature was 5 - 10 ºC higher for the immobilized lipase. Higher yields in the butyl butyrate synthesis were found for the derivatives prepared by activation with glycidol and epichlorohydrin.

Keywords : covalent attachment; lipase; organic supports.

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