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Química Nova

versão impressa ISSN 0100-4042

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MORIOKA, Luiz Rodrigo Ito; MATOS, Ângelo Paggi; OLIVO, Gisele  e  SANT'ANNA, Ernani Sebastião. Evaluation of flocculation and lipid extraction of Chlorella sp. cultivated in concentrated desalination. Quím. Nova [online]. 2014, vol.37, n.1, pp.44-49. ISSN 0100-4042.  http://dx.doi.org/10.1590/S0100-40422014000100009.

Chlorella sp. was used to assess algal lipid production with concentrated desalination. In order to investigate the action of the flocculating agent calcium chloride and pH, a Box-Behnken Design and a Central Composite Design (CCD) were carried out. Also, Soxhlet and Supercritical Fluid Extraction (SFE), with and without sonication lipid extraction methods, were examined. The optimal flocculation conditions were pH 10.0 and 2.0 g/L of calcium chloride concentration. The highest lipid content of Chlorella sp. was obtained using the Soxhlet extraction method. The most abundant fatty acid extracted by Soxhlet and SFE, with and without sonication, was palmitic acid, whose proportions were 57.4%, 35.3% and 25.5%, respectively.

Palavras-chave : concentrated desalination; flocculation; microalga oil.

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